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human cell lines u266  (DSMZ)


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    Structured Review

    DSMZ human cell lines u266
    Human Cell Lines U266, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cell+lines+u266/U-266/pm41545347-41-27-38
    Average 95 stars, based on 264 article reviews
    human cell lines u266 - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Expressing:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Marker:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Control:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Western Blot:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    ChIP-sequencing:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Binding Assay:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Activation Assay:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Labeling:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Fluorescence:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Conjugation Assay:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Translocation Assay:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Membrane:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Molecular Weight:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    MTT Assay:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Clinical Proteomics:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Derivative Assay:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Immunohistochemistry:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Staining:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Solvent:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Two Tailed Test:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Phospho-proteomics:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Real-time Polymerase Chain Reaction:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Plasmid Preparation:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Co-Culture Assay:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Isolation:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Cell Culture:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Incubation:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.

    Lysis:

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival.
    Article Snippet: Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.Pharmacological inhibition of either CREB1 or TXNIP synergizes with proteasome inhibitors, offering a novel therapeutic strategy for patients with MM.. METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: Regulation of stress tolerance by CREB1 sustains multiple myeloma cell survival
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266, JJN-3, and OPM-2 were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ).. KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.KMS-11 and KMS-11 BTZ were provided by Dr. Arianna Giacomini, University of Brescia.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma
    Article Snippet: The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DSMZ).. Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.Human cell lines KMS-11 and KMS-11 BTZ were kindly provided by Dr. Arianna Giacomini, University of Brescia, Italy.

    Article Title: CREB1 promotes expression of immune checkpoint HLA-E leading to immune escape in multiple myeloma.
    Article Snippet: These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.These findings highlight the role of CREB1 in immune escape, leading to novel therapeutic approaches for treating patients with MM.. MATERIALS AND METHODS Cell lines and cultures The human cell lines H929, RPMI-8226, MM.1S, MM.1R, and HEK293T cells were purchased from the American Type Culture Collection (ATCC), while the human cell lines U266 and OPM-2 were purchased from the Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures Received: 8 January 2024 Revised: 7 May 2024 Accepted: 6 June 2024 1Division of Hematology, Department of Internal Medicine, The Ohio State University College of Medicine, Columbus, OH, USA.. 2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.2Comparative Pathology and Digital Imaging Shared Resource Main Laboratory, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA.



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    a MSP gel electrophoresis showing methylation (M) and unmethylation (U) status in representative samples. b RASD1 mRNA expression in U266 cells following DAC treatment. c Quantification of apoptosis rates (mean ± SD) in U266 cells (** P < 0.01). d Representative flow cytometry plots of apoptosis (Annexin V-YF647A/PI staining); Total apoptosis: 5.04% in the control group and 12.08% in the DAC-treated group

    Journal: Blood Research

    Article Title: Hypermethylation-mediated silencing of RASD1 drives multiple myeloma pathogenesis

    doi: 10.1007/s44313-026-00125-6

    Figure Lengend Snippet: a MSP gel electrophoresis showing methylation (M) and unmethylation (U) status in representative samples. b RASD1 mRNA expression in U266 cells following DAC treatment. c Quantification of apoptosis rates (mean ± SD) in U266 cells (** P < 0.01). d Representative flow cytometry plots of apoptosis (Annexin V-YF647A/PI staining); Total apoptosis: 5.04% in the control group and 12.08% in the DAC-treated group

    Article Snippet: The human MM cell line U266 was obtained from the American Type Culture Collection, and was maintained in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum.

    Techniques: Nucleic Acid Electrophoresis, Methylation, Expressing, Flow Cytometry, Staining, Control

    Identification of constructed TCF4−/+ U266 and MMSA−1−/+ U266 stable cell lines and MMSA-1 mRNA and protein levels in TCF4−/+ U266 cells 1: U266 cells; 2: TCF4− U266-NC; 3. TCF4− U266 cells; 4: TCF4+ U266-NC; 5. TCF4+ U266 cells

    Journal: Annals of Hematology

    Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

    doi: 10.1007/s00277-026-06740-8

    Figure Lengend Snippet: Identification of constructed TCF4−/+ U266 and MMSA−1−/+ U266 stable cell lines and MMSA-1 mRNA and protein levels in TCF4−/+ U266 cells 1: U266 cells; 2: TCF4− U266-NC; 3. TCF4− U266 cells; 4: TCF4+ U266-NC; 5. TCF4+ U266 cells

    Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

    Techniques: Construct, Stable Transfection

    Dual-Luciferase Report Assay results in different U266 cells

    Journal: Annals of Hematology

    Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

    doi: 10.1007/s00277-026-06740-8

    Figure Lengend Snippet: Dual-Luciferase Report Assay results in different U266 cells

    Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

    Techniques: Luciferase

    MMSA-1 and RAS co-localization and interaction in U266 cells 1: U266 cells; 2: MMSA−1− U266-NC; 3. MMSA−1− U266 cells; 4: MMSA−1+ U266-NC; 5. MMSA−1+ U266 cells

    Journal: Annals of Hematology

    Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

    doi: 10.1007/s00277-026-06740-8

    Figure Lengend Snippet: MMSA-1 and RAS co-localization and interaction in U266 cells 1: U266 cells; 2: MMSA−1− U266-NC; 3. MMSA−1− U266 cells; 4: MMSA−1+ U266-NC; 5. MMSA−1+ U266 cells

    Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

    Techniques:

    RAS downstream related molecule expressions in different U266 cells 1: U266; 2: MMSA−1− U266-NC; 3. MMSA−1− U266; 4: MMSA−1+ U266-NC; 5. MMSA−1 + U266

    Journal: Annals of Hematology

    Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

    doi: 10.1007/s00277-026-06740-8

    Figure Lengend Snippet: RAS downstream related molecule expressions in different U266 cells 1: U266; 2: MMSA−1− U266-NC; 3. MMSA−1− U266; 4: MMSA−1+ U266-NC; 5. MMSA−1 + U266

    Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

    Techniques:

    MMSA-1’s effect on U266 cells proliferation, migration and apoptosis A showed cloning formation assay result; B showed transwell assay result; C showed apoptosis related proteins expression and D showed mitochondrial apoptosis in different U266 cells. 1: U266; 2: U266 + CCCP-1; 3. MMSA−1− U266-NC; 4. MMSA−1− U266-NC + CCCP-1; 5. MMSA−1− U266; 6: MMSA−1− U266 + CCCP-1; 7. MMSA−1+ U266-NC; 8. MMSA−1+ U266-NC + CCCP-1; 9. MMSA−1 + U266; 10. MMSA−1 + U266 + CCCP-1

    Journal: Annals of Hematology

    Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

    doi: 10.1007/s00277-026-06740-8

    Figure Lengend Snippet: MMSA-1’s effect on U266 cells proliferation, migration and apoptosis A showed cloning formation assay result; B showed transwell assay result; C showed apoptosis related proteins expression and D showed mitochondrial apoptosis in different U266 cells. 1: U266; 2: U266 + CCCP-1; 3. MMSA−1− U266-NC; 4. MMSA−1− U266-NC + CCCP-1; 5. MMSA−1− U266; 6: MMSA−1− U266 + CCCP-1; 7. MMSA−1+ U266-NC; 8. MMSA−1+ U266-NC + CCCP-1; 9. MMSA−1 + U266; 10. MMSA−1 + U266 + CCCP-1

    Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

    Techniques: Migration, Cloning, Tube Formation Assay, Transwell Assay, Expressing

    Adhesion molecules and angiogenesis factors levels in different U266 cells A showed qRT-PCR result and B showed Western Blot result of adhesion molecules; C showed angiogenesis factors levels. 1: U266 cells, 2: MMSA−1− U266-NC; 3. MMSA−1− U266; 4. MMSA−1+ U266-NC; 5. MMSA−1+ U266

    Journal: Annals of Hematology

    Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

    doi: 10.1007/s00277-026-06740-8

    Figure Lengend Snippet: Adhesion molecules and angiogenesis factors levels in different U266 cells A showed qRT-PCR result and B showed Western Blot result of adhesion molecules; C showed angiogenesis factors levels. 1: U266 cells, 2: MMSA−1− U266-NC; 3. MMSA−1− U266; 4. MMSA−1+ U266-NC; 5. MMSA−1+ U266

    Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

    Techniques: Quantitative RT-PCR, Western Blot

    BBR decreases IgE secretion but not IgG secretion. The expression of IgE by IgE-producing myeloma cells, U266, was determined by ELISA following berberine (BBR) treatment at 10, 5, and 2.5 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL) for 6 days ( A ). The expression of IgG by IgG-producing myeloma cells, ARH-77, was determined by ELISA, treated as U266 ( B ). N = 3–4, ** p ≤ 0.01, *** p ≤ 0.0001, ns = not significant. Each dot represents a single biological replicate.

    Journal: Cells

    Article Title: A Small Molecule Compound, Berberine Reduces IgE but Not IgG Production via Promoting miRNA-34a-p53 Axis

    doi: 10.3390/cells14221799

    Figure Lengend Snippet: BBR decreases IgE secretion but not IgG secretion. The expression of IgE by IgE-producing myeloma cells, U266, was determined by ELISA following berberine (BBR) treatment at 10, 5, and 2.5 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL) for 6 days ( A ). The expression of IgG by IgG-producing myeloma cells, ARH-77, was determined by ELISA, treated as U266 ( B ). N = 3–4, ** p ≤ 0.01, *** p ≤ 0.0001, ns = not significant. Each dot represents a single biological replicate.

    Article Snippet: The IgE-producing human myeloma U266 cell line and IgG-producing human myeloma ARH-77 cell lines were obtained from American Type Culture Collection, (ATCC, Rockville, MD, USA).

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay

    BBR does not affect the viability of IgE or IgG producing cells. ( A ) The viability of IgE by IgE-producing myeloma cells, U266, was determined by trypan blue exclusion assay, following berberine (BBR) treatment at 10, 5, and 2.5 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL) for 6 days. ( B ) The same was performed for IgG-producing myeloma cells, ARH-77. ( C ) Total cell count determined by Trypan Blue was used to determine total cells per mL of U266 cells and ( D ) ARH-77 cells. N = 3. BBR= Berberine. * p ≤ 0.05, ** p ≤ 0.01, ns = not significant. Each dot represents a single biological replicate.

    Journal: Cells

    Article Title: A Small Molecule Compound, Berberine Reduces IgE but Not IgG Production via Promoting miRNA-34a-p53 Axis

    doi: 10.3390/cells14221799

    Figure Lengend Snippet: BBR does not affect the viability of IgE or IgG producing cells. ( A ) The viability of IgE by IgE-producing myeloma cells, U266, was determined by trypan blue exclusion assay, following berberine (BBR) treatment at 10, 5, and 2.5 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL) for 6 days. ( B ) The same was performed for IgG-producing myeloma cells, ARH-77. ( C ) Total cell count determined by Trypan Blue was used to determine total cells per mL of U266 cells and ( D ) ARH-77 cells. N = 3. BBR= Berberine. * p ≤ 0.05, ** p ≤ 0.01, ns = not significant. Each dot represents a single biological replicate.

    Article Snippet: The IgE-producing human myeloma U266 cell line and IgG-producing human myeloma ARH-77 cell lines were obtained from American Type Culture Collection, (ATCC, Rockville, MD, USA).

    Techniques: Trypan Blue Exclusion Assay, Concentration Assay, Cell Counting

    BBR affects proliferation of IgE-producing myeloma cells with no effect on IgG-producing myeloma cells. ( A ) The proliferation of IgE by IgE-producing myeloma cells, U266, was determined by XTT assay, following berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL) for 6 days. ( B ) The same was performed for IgG-producing myeloma cells, ARH-77. N = 3. BBR = berberine, DMSO = dimethyl sulfoxide. * p ≤ 0.05, ns = not significant. Each dot represents a single biological replicate.

    Journal: Cells

    Article Title: A Small Molecule Compound, Berberine Reduces IgE but Not IgG Production via Promoting miRNA-34a-p53 Axis

    doi: 10.3390/cells14221799

    Figure Lengend Snippet: BBR affects proliferation of IgE-producing myeloma cells with no effect on IgG-producing myeloma cells. ( A ) The proliferation of IgE by IgE-producing myeloma cells, U266, was determined by XTT assay, following berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL) for 6 days. ( B ) The same was performed for IgG-producing myeloma cells, ARH-77. N = 3. BBR = berberine, DMSO = dimethyl sulfoxide. * p ≤ 0.05, ns = not significant. Each dot represents a single biological replicate.

    Article Snippet: The IgE-producing human myeloma U266 cell line and IgG-producing human myeloma ARH-77 cell lines were obtained from American Type Culture Collection, (ATCC, Rockville, MD, USA).

    Techniques: XTT Assay, Concentration Assay

    BBR affects cell cycle genes of IgE-producing myeloma cells with no effect on IgG-producing myeloma cells. ( A ) qRT-PCR of IgE heavy chain ( IGHE ) and cell cycle genes cyclin D1 ( CCND1 ), tumor protein 53 ( TP53 ), and growth arrest and DNA-damage inducible alpha ( GADD45A ), in IgE-producing U266 were performed after 6 days of berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL). qPCR analysis of cell cycle proteins of IgE-producing U266 was performed after 6 days of berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL) and data were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression. ( B ) The same was performed for ARH-77, with analysis of IgG heavy chain ( IGHG ). N = 3. * p ≤ 0.05, ** p ≤ 0.01, ns = not significant. Each dot represents a single biological replicate.

    Journal: Cells

    Article Title: A Small Molecule Compound, Berberine Reduces IgE but Not IgG Production via Promoting miRNA-34a-p53 Axis

    doi: 10.3390/cells14221799

    Figure Lengend Snippet: BBR affects cell cycle genes of IgE-producing myeloma cells with no effect on IgG-producing myeloma cells. ( A ) qRT-PCR of IgE heavy chain ( IGHE ) and cell cycle genes cyclin D1 ( CCND1 ), tumor protein 53 ( TP53 ), and growth arrest and DNA-damage inducible alpha ( GADD45A ), in IgE-producing U266 were performed after 6 days of berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL). qPCR analysis of cell cycle proteins of IgE-producing U266 was performed after 6 days of berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL) and data were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression. ( B ) The same was performed for ARH-77, with analysis of IgG heavy chain ( IGHG ). N = 3. * p ≤ 0.05, ** p ≤ 0.01, ns = not significant. Each dot represents a single biological replicate.

    Article Snippet: The IgE-producing human myeloma U266 cell line and IgG-producing human myeloma ARH-77 cell lines were obtained from American Type Culture Collection, (ATCC, Rockville, MD, USA).

    Techniques: Quantitative RT-PCR, Concentration Assay, Expressing

    BBR affects cell cycle genes of IgE-producing myeloma cells with no effect on IgG-producing myeloma cells. ( A ) Western Blot analysis of cell cycle genes in IgE-producing U266 was performed after 6 days of berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL). ( B ) The same was performed for ARH-77. N = 3. CDK2 = cyclin dependent protein kinase 2, CDKN1C = cyclin dependent kinase inhibitor 1C, TP53 = tumor protein 53. * p ≤ 0.05, ** p ≤ 0.01, ns = not significant. Each dot represents a single biological replicate.

    Journal: Cells

    Article Title: A Small Molecule Compound, Berberine Reduces IgE but Not IgG Production via Promoting miRNA-34a-p53 Axis

    doi: 10.3390/cells14221799

    Figure Lengend Snippet: BBR affects cell cycle genes of IgE-producing myeloma cells with no effect on IgG-producing myeloma cells. ( A ) Western Blot analysis of cell cycle genes in IgE-producing U266 was performed after 6 days of berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL). ( B ) The same was performed for ARH-77. N = 3. CDK2 = cyclin dependent protein kinase 2, CDKN1C = cyclin dependent kinase inhibitor 1C, TP53 = tumor protein 53. * p ≤ 0.05, ** p ≤ 0.01, ns = not significant. Each dot represents a single biological replicate.

    Article Snippet: The IgE-producing human myeloma U266 cell line and IgG-producing human myeloma ARH-77 cell lines were obtained from American Type Culture Collection, (ATCC, Rockville, MD, USA).

    Techniques: Western Blot, Concentration Assay

    BBR affects expression of miRNAs involved in modulation of the p53 pathway of IgE-producing myeloma cells with no effect on IgG-producing myeloma cells. ( A ) miRNA isolation and qPCR analysis of miRNAs (miR) implicated IgE-regulation, miR-155, miR-143, miR-34a, and miR-146a were performed on U266 after 6 days of berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL). ( B ) The same was performed for ARH-77. N = 3. * p ≤ 0.05, ** p ≤ 0.01, ns = not significant. Each dot represents a single biological replicate.

    Journal: Cells

    Article Title: A Small Molecule Compound, Berberine Reduces IgE but Not IgG Production via Promoting miRNA-34a-p53 Axis

    doi: 10.3390/cells14221799

    Figure Lengend Snippet: BBR affects expression of miRNAs involved in modulation of the p53 pathway of IgE-producing myeloma cells with no effect on IgG-producing myeloma cells. ( A ) miRNA isolation and qPCR analysis of miRNAs (miR) implicated IgE-regulation, miR-155, miR-143, miR-34a, and miR-146a were performed on U266 after 6 days of berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL). ( B ) The same was performed for ARH-77. N = 3. * p ≤ 0.05, ** p ≤ 0.01, ns = not significant. Each dot represents a single biological replicate.

    Article Snippet: The IgE-producing human myeloma U266 cell line and IgG-producing human myeloma ARH-77 cell lines were obtained from American Type Culture Collection, (ATCC, Rockville, MD, USA).

    Techniques: Expressing, Isolation, Concentration Assay